PICOLAY Kurzreferenz (Deutsch) | Short reference (English) Main window | Image window | Result window | 3D settings 3D settings Length of Z axis (% of image height) This parameter defines the depth of the object to be displayed (from top-most to lowest frame of your stack). To avoid calculations in µm or pixels, the value is given as percent of the Y-axis (i.e. image height). Higher values give more depth to the object and vice versa. Enlarge pixels Enlarges pixels in order to prevent the occurrence of fissures in 3D-images. The latter arise as the surface area of 3-D images is larger than that of the (flat) stacked image. The value has to be increased for objects with high depth. Perspective (%) A value > 0 has the effect that close parts of the displayed object are enlarged compared to those in the background. This is not normally necessary for a 3-D impression. Too high values will distort the object. Projection based on depth map Standard setting, uses (together with the stacked image) the depth map for the generation of spatial and 3-D projections. Hologram stacking Allows for displaying structures of multilayered objects that are hidden when depth-map based is performed. One has to set a rotation angle, filter size an minimum contrast prior to analysing the stack again. Back Closes the 3-D view window. Go Starts the generation of rotations and 3-D images. Images to be generated 3-D view For stereoscopic viewing two images (for the left and right eye) are required. However, one can also rotate an object without 3-D. Viewing angle Describes the viewing angle between your eyes when you look at your monitor or at a big screen. For short observation distances use large value (3 - 4°), for long distances (> 3 m Abstand) small values (e.g., 2°). Red-cyan Generates an anaglyph image for red-cyan goggles, left eye red, right eye cyan. [RLR] or [LRL] = Right-Left-Right or Left-Right-Left Generates a 3-D image, with the object shown three times for the right and left eye in alternating order. The result can be received by both, parallel- and cross-viewing, and gives both a convex and concave impression. [R] + [L] Produces to separate images for the left and right eye. These can be used for the generation of MPO files and other purposes. Rocking gif Generates an animated gif image, showing the left- and right-eye image flickering with the frequency set under Options (main window). A rocking gif provides a spatial impression to obervers who can not perform parallel- and cross-viewing. [RL] + [LR] Generates various steroscopic images for different purposes, for cross- [RL] or parallel viewing [LR], side by side or one on top of the other [--], interlaced or as jps file. [RL] [LR] Gerates a 3-D images composed of an [RL]- and an [LR]-images one on top of the other. The result can be received by both, parallel- and cross-viewing, and gives both a convex and concave impression. Background Average Uses the average of all images as background for low-contrast areas. To be used for small rotatin angles. Colour Uses a selected colour as background for low-contrast areas. To be used for large rotation angles. To select the colour RIGHT-click into the image window (pipette function). 3-D rotation parameters X, Y and Z Gives the projection angle of the three axes in the range of 0.0 - 360.0°. Stepwise rotation around axis: Select the axis for stepwise rotation. Step Defines the degree of rotatiopn per step, allow values < 1°. Repeats Gives the number of rotation steps. Hologram parameters Based on sharp areas Makes visible all structures that reach the adjusted minimum contrast. Filter radius Defines the size of the search mask for structures. Minimum contrast Defines the contrast threshold at which structures will be visualised. A too small step will display unsharp areas hiding underlying structures. Large values will make low-contrast structures invisible. Based on target colour Generates a hologram based on the intensity of a selected colour (mayvbe a fluorescence dye etc.) not regarding contrast. To select the colour perform a RIGHT-click into the original image as described above. Top of page